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embryonic mhc  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank embryonic mhc
    Embryonic Mhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryonic+mhc/anti-Myosin+heavy+chain/pmc13040700-188-51-55
    Average 96 stars, based on 19 article reviews
    embryonic mhc - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunopeptidomics:

    Article Title: Hsp70 Interacts with Mitogen-Activated Protein Kinase (MAPK)-Activated Protein Kinase 2 To Regulate p38MAPK Stability and Myoblast Differentiation during Skeletal Muscle Regeneration
    Article Snippet: Antibodies from Cell Signaling Technology were 405 the following: anti-pp38MAPK (#4511), anti-MKK3 (#8535), anti-MKK6 (#8550), anti-ASK 406 (#3762), anti-AKT, anti-PI3K, anti-MK2 (#7421), anti-MK3 (#3043), anti-JNK (#9252). .. 407 Antibodies from Developmental Studies Hybridoma Bank were the following: anti-MHC1E 408 (MF-20), anti-myogenin (F5D), Pax7 (Pax7-s), embryonic MHC (BF-G6-c). .. Other antibodies 409 were the following: anti-FLAG epitope (Sigma-Aldrich, F7425), anti-HA epitope (Invitrogen, 410 715500), anti-Tubulin (HuaAn Biotechnology), anti-Hsc70 (SPA815 Stressgen), anti-HSF1 411 (SPA901, Stressgen), anti-KIF5B (Abcam; ab42492), anti-JLP (Abcam, ab12331), anti-CDO 412 (R&D systems), anti-pHsp27 (Ser78) (Millipore, 04-447).

    Article Title: Functional and structural pathologies in skeletal muscle of a rat model of Duchenne muscular dystrophy
    Article Snippet: .. The following primary antibodies were used for immunofluorescence in the present study: dystrophin (1:100; MANEX1011B, Clone 1C7; DSHB), β-dystroglycan (1:100; MANDAG2, Clone 7A11; DSHB), utrophin (1:50; VP-U579; Vector Laboratories), α-sarcoglycan (1:50; VP-A105; Vector Laboratories); syntrophins (1:2000; #11425; Abcam), perilipin 1 (1:500; #9349; Cell Signaling Technology), anti-Laminin β2 antibody (1:10,000; [ ]), embryonic MHC (1:100; F1.652; DSHB), type I myofibers (1:50; BA-D5; DSHB), Type IIa myofibers (1:50; SC-71; DSHB), type IIx myofibers (1:20; 6H1; DSHB), pan-muscle MHC(1:100; MF 20; DSHB), COMP (1:800; #NBP2-92658; Noveus Biologicals), α-SMA (1:1000; #7817; Abcam), SMOC2 (1:500; #AF5140; R&D Systems), and PDGFRα (1:500; #AF1062; R&D Systems). .. Image acquisition was performed with a Leica Application Suite X software on a Leica STELLARIS 5 confocal system.

    Article Title: Tcf12 is required to sustain myogenic genes synergism with MyoD by remodelling the chromatin landscape
    Article Snippet: For western blotting, MuSCs were digested using 0.25% trypsin and lysed using RIPA buffer, and total protein concentration was measured using a BCA kit (Beyotime, P0010S). .. The following antibodies were used: Pax7 (DSHB, 1:100), myosin heavy chain (MHC; DSHB, 1:400), embryonic MHC (eMHC; DSHB, 1:500), laminin (Sigma, 1:500) and TCF12 (Abclonal, 1:300). ..

    Article Title: Preclinical quality, safety, and efficacy of a CGMP iPSC-derived myogenic progenitor product for the treatment of muscular dystrophies.
    Article Snippet: Karim Azzag,1,13 Alessandro Magli,1,2,13 James Kiley,1 Darin Sumstad,3 Diane Kadidlo,3 Sarah B. Crist,1 Beverly Norris,4 Aaron Ahlquist,1 Laura L. Hocum Stone,5 John Everett,6 Jill Shappa Faustich,5 Davis Seelig,7,8 Parthasarathy Rangarajan,5 Hyunkee Kim,1 Craig Flory,4 Frederic Bushman,6 Sabarinathan Ramachandran,2,5 Peter B. Kang,9,10 Robert J. Schumacher,4 John E. Wagner,2,11 Michael Kyba,2,9,11 Melanie L. Graham,5,12 David H. McKenna,2,3 and Rita C.R.. Perlingeiro1,2,9

    Article Title: SU9516 Increases α7β1 Integrin and Ameliorates Disease Progression in the mdx Mouse Model of Duchenne Muscular Dystrophy.
    Article Snippet: 10-mm sections of diaphragm and gastrocnemius muscles frommice were immunostained using antibodies against a7B integrin and b1D as described previously,25 followed by fluorescein isothiocyanate (FITC) donkey anti-rabbit (1:1,000, Jackson ImmunoResearch) incubation. .. Embryonic MHC (mAb 1:50, DSHB) overnight incubation at 4 C was performed on cryosections, followed by 1-hr incubation with Alexa Fluor 488-conjugated secondary antibody (1:200, Invitrogen). ..

    Article Title: Hsp70 Interacts with Mitogen-Activated Protein Kinase (MAPK)-Activated Protein Kinase 2 To Regulate p38MAPK Stability and Myoblast Differentiation during Skeletal Muscle Regeneration
    Article Snippet: Antibodies from Cell Signaling Technology included the following: anti-pp38MAPK (4511), anti-MKK3 (8535), anti-MKK6 (8550), anti-ASK (3762), anti-AKT, anti-PI3K, anti-MK2 (7421), anti-MK3 (3043), and anti-JNK (9252). .. Antibodies from Developmental Studies Hybridoma Bank included the following: anti-MHC1E (MF-20), antimyogenin (F5D), Pax7 (Pax7-s), and embryonic MHC (BF-G6-c). .. Images were analyzed with Zeiss LSM Image Examiner software or FV10-ASW 3.0 viewer.

    Immunofluorescence:

    Article Title: Functional and structural pathologies in skeletal muscle of a rat model of Duchenne muscular dystrophy
    Article Snippet: .. The following primary antibodies were used for immunofluorescence in the present study: dystrophin (1:100; MANEX1011B, Clone 1C7; DSHB), β-dystroglycan (1:100; MANDAG2, Clone 7A11; DSHB), utrophin (1:50; VP-U579; Vector Laboratories), α-sarcoglycan (1:50; VP-A105; Vector Laboratories); syntrophins (1:2000; #11425; Abcam), perilipin 1 (1:500; #9349; Cell Signaling Technology), anti-Laminin β2 antibody (1:10,000; [ ]), embryonic MHC (1:100; F1.652; DSHB), type I myofibers (1:50; BA-D5; DSHB), Type IIa myofibers (1:50; SC-71; DSHB), type IIx myofibers (1:20; 6H1; DSHB), pan-muscle MHC(1:100; MF 20; DSHB), COMP (1:800; #NBP2-92658; Noveus Biologicals), α-SMA (1:1000; #7817; Abcam), SMOC2 (1:500; #AF5140; R&D Systems), and PDGFRα (1:500; #AF1062; R&D Systems). .. Image acquisition was performed with a Leica Application Suite X software on a Leica STELLARIS 5 confocal system.

    other:

    Article Title: Functional and structural pathologies in skeletal muscle of a rat model of Duchenne muscular dystrophy.
    Article Snippet: The Band signal intensities were measured using Image Studio Lite software (Li-Cor Biosciences), normalized to sample loading (Ponceau S stain), and reported relative to respective control samples.

    Article Title: Functional and structural pathologies in skeletal muscle of a rat model of Duchenne muscular dystrophy
    Article Snippet: Protein extraction and immunoblotting were performed on snap-frozen heart and gastrocnemius muscles, as previously described [ – ], using the following primary antibodies: dystrophin [1:100; MANHINGE1B, Clone 10F9; Developmental Studies Hybridoma Bank (DSHB)], dystrophin (1:100; MANEX1011B; Clone 1C7; DSHB), dystrophin (1:100; MANEX1011C; Clone 4F9; DSHB), β-dystroglycan (1:1000; 11017-1-AP; ProteinTech), dystrobrevin (1:1000; #610766; BD Biosciences), syntrophins (1:2000; #11425; Abcam), and perilipin 1 (1:500; #9349; Cell Signaling Technology).

    Incubation:

    Article Title: SU9516 Increases α7β1 Integrin and Ameliorates Disease Progression in the mdx Mouse Model of Duchenne Muscular Dystrophy.
    Article Snippet: 10-mm sections of diaphragm and gastrocnemius muscles frommice were immunostained using antibodies against a7B integrin and b1D as described previously,25 followed by fluorescein isothiocyanate (FITC) donkey anti-rabbit (1:1,000, Jackson ImmunoResearch) incubation. .. Embryonic MHC (mAb 1:50, DSHB) overnight incubation at 4 C was performed on cryosections, followed by 1-hr incubation with Alexa Fluor 488-conjugated secondary antibody (1:200, Invitrogen). ..



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